Fig. 5

Effects of ALCAR on pro-angiogenic factors and endothelial cell chemokine receptors. PCa (PC-3, DU-145, LNCaP) and BPH were treated with ALCAR 1 and 10 mM for 24 h and analysed for pro-angiogenic cytokine production by flow cytometry. ALCAR 1 and 10 mM reduced the expression of (a) VEGF and (b) CXCL8. Conditioned media (CM) from PCa (PC-3, DU-145, LNCaP) and BPH cell lines were collected following 24 h of treatment with ALCAR 1 and 10 mM. Human Umbilical Vein Endothelial Cells (HUVEC) were treated with 50 μg/ml of CM from PCa (PC-3, DU-145, LNCaP) and BPH cell line and assessed by flow cytometry for the expression the chemokine receptors (c) CXCR4, (d) CXCR1, (e) CXCR2 (f) CCR2. (g) Representative heatmap for the investigated chemokine receptors as fold-change over non treated cells (0). Results are showed as normalised over non treated cells (0), as mean ± SEM, ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001