Fig. 6

Indirect effects of ALCAR on capillary-like structure formation of HUVECs. Secretome profiling on PCa cell lines in response to ALCAR treatment by BIOPLEX analyses showing ALCAR ability to limit (a) VEGF, (b) CXCL8 and (c) Angiogenin (ANG) release on PCa cell lines (PC-3, DU-145, LNCaP) following 24 h of treatments with ALCAR 1 mM. (d, e) Morphogenesis assay on HUVECs pre-treated with 50 μg/ml of CM collected from DU-145 following 24 h of treatment with ALCAR 1 and 10 mM. (d) Representative images of tubular structures photographed at 5× magnification and (e) quantified by the Angiogenesis analyser ImageJ tool kit. HUVEC exposed to CM from DU-145 treated with ALCAR 1 and 10 mM exhibit a reduced ability to form capillary-like structures on Matrigel. SFM: cells cultured in serum-free EBM-2 medium; FBS+: cell treated with complete EGM-2 medium. Data are showed as Mean ± SEM. **p < 0.01, ***p < 0.001, ****p < 0.0001