Fig. 3

NSD1 regulates H3K27me3 methylation in the Wnt10b promoter region to promote Wnt10B transcription. a, Wnt10b expression in adjacent normal and HCC tissues identified by immunohistochemical staining (× 400). b, Protein expression of Wnt10b normalized to GAPDH in adjacent normal and HCC tissues (n = 63) determined by Western blot analysis. c, Correlation analysis between expression of NSD1 and Wnt10b in HCC. d, Protein expression of Wnt10b normalized to GAPDH in HCC cells following NSD1 knockout determined by Western blot analysis. e, Protein expression of H3K36me2, H3K27me2, H3K27me3 in cells after knockout of NSD1 determined by Western blot analysis. f, Enrichment of H3K27me3 in Wnt10b promoter region in cells after knockout of NSD1 detected by ChIP assay. * p < 0.05 vs. adjacent normal tissues, or the blank group (HepG2/SK-Hep1 cells without any treatment). Data (mean ± s.d.) between two groups were analyzed by paired t test, and those among multiple groups were analyzed by one-way ANOVA, followed by Tukey’s post-hoc test, while data at different time points were analyzed by repeated measures ANOVA, followed by the Bonferroni’s post-hoc test. The experiment was repeated 3 times independently