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Table 2 Primer sequences for RT-qPCR

From: CRISPR/Cas9-mediated knockout of NSD1 suppresses the hepatocellular carcinoma development via the NSD1/H3/Wnt10b signaling pathway

GenesPrimer sequences
NSD1F: 5′-AGGTGTAGAACACGATCCCG-3′
R: 5′-AGCCGACCTTTAGATGCAGA-3’
Wnt10bF: 5′-CACTGGAGGTCCTGATCGATC-3’
R: 5′-CAGCCCCAAGGTAAGGCTGAC-3’
Wnt10b-promoterF: 5′-TTTTGGATCCCAAGGCCCTC-3’
R: 5′-GTTTGGCCCTAGCAGAGGTT-3’
GAPDHF: 5′-GAAGGTGAAGGTCGGAGTC-3’
R: 5′-GAAGATGGTGATGGGATTTC-3’
  1. Note: F Forward, R Reverse, RT-qPCR Reverse transcription quantitative polymerase chain reaction, NSD1 Nuclear receptor binding SET domain protein 1, Wnt10b Wingless-related mouse mammary tumor virus integration site 10b, GAPDH Glyceraldehyde-3-phosphate dehydrogenase