Fig. 2
Suppression of NOTCH2 promotes NPC cell metastasis. a shNOTCH2 cells were established. Western blotting confirmed the knockdown of NOTCH2 in CNE-2 and 5-8F cells. b Wound healing assays showed that knockdown of NOTCH2 increased the CNE-2 and 5-8F cell migration distance. Right panel: Quantification of the gap closure. c Transwell migration assays showed that knockdown of NOTCH2 increased the CNE-2 and 5-8F cell migration. Transwell invasion assays showed that knockdown of NOTCH2 increased the CNE-2 and 5-8F cell invasion. Data are the mean ± SD of at least three independent experiments; * P < 0.05, Student’s t-test. d The NOTCH2KO group had significantly more nude mice with lung metastasis than the Ctrl group. First and second panel: Representative hematoxylin and eosin (H&E) staining of the lungs. Third panel: Quantification of nude mice with lung metastasis. Scale bar = 50 mm. P-values were calculated using the χ2 test. e Mice in the NOTCH2KO group had significantly more metastatic lesions on the liver surface than mice in the Ctrl group. First panel: Macroscopic metastatic nodules on the surface of the liver (scale bar = 1 cm). Second and third panel: Representative H&E staining of the livers; scale bar = 50 mm. Fourth panel: Quantification of metastases on the liver surface. P-values were calculated using Student’s t-test. Arrows indicate surface metastatic nodules