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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: NOTCH2 negatively regulates metastasis and epithelial-Mesenchymal transition via TRAF6/AKT in nasopharyngeal carcinoma

Fig. 3

Overexpression of NOTCH2 inhibits NPC cell metastasis. a oeNOTCH2 cells were established. Western blotting confirmed the overexpression of NOTCH2 in CNE-2 and 5-8F cells. b Wound healing assays showed that overexpression of NOTCH2 inhibited the CNE-2 and 5-8F cell migration distance. Right panel: Quantification of the gap closure. c Transwell migration assays showed that overexpression of NOTCH2 inhibited the CNE-2 and 5-8F cell migration. Transwell invasion assays showed that overexpression of NOTCH2 inhibited the CNE-2 and 5-8F cell invasion. Data are the mean ± SD of at least three independent experiments; *P < 0.05, Student’s t-test. d The oeNOTCH2 and oeVec groups did not develop lung metastasis. Representative H&E staining of the lung is shown. Scale bar = 50 mm. e The oeNOTCH2 group had significantly fewer metastatic lesions on the liver surface than the oeVec group. First panel: Macroscopic metastatic nodules on the surface of the liver (scale bar = 1 cm). Second and third panel: Representative H&E staining of the liver (scale bar = 50 mm). Fourth panel: Quantification of metastases on the liver surface. P-values were calculated using Student’s t-test. Arrows indicate surface metastatic nodules

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