Fig. 1

Arf6-KD accumulated CD147 in the endomembrane compartments. Arf6-perturbed liver cancer cells were incubated with the H18Ab-AF488 complex at 37 °C for 15 min uptake, then quickly rinsed with PBS/1 M NaCl, fixed, permeabilized, stained by anti-HA Ab, and visualized under a confocal microscope. a Representative observations are shown. Scale bar: 20 um. b Box-and-whiskers plots depict the uptake of the H18Ab-AF488 complex in cell populations. The CD147 uptake in untreated cells (WT) was set as 100%. Significant differences compared with NC-KD cells are shown. c Uptake of the H18Ab-AF488 complex in Arf6-perturbed cells was quantified by flow cytometry. n = 3. HG-, and LG-CD147 were blotted from the endomembrane fractions of Arf6-perturbed liver cancer cells. Representative results from three independent experiments are shown (d, e, f), and corresponding quantitative scans of CD147 blots were analyzed (g, h, i). The CD147 level in untreated cells (WT) was set as 100%. Significant differences compared with NC-KD cells are shown. n = 3. * P < 0.05, ** P < 0.01