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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Arf6-driven endocytic recycling of CD147 determines HCC malignant phenotypes

Fig. 3

Arf6-KD impaired the activation of Rab5 and Rab22. Arf6-perturbed liver cancer cells were serum-starved, and stimulated with HGF-contained medium, lysed immediately to extract membrane fractions, and incubated with GST-EEA and -Rabenosyn-5 beads, respectively. Activated Rab GTPases in pull-down precipitates were blotted with corresponding Abs. Representative blot results from three independent experiments are shown (a, b, c). Activated Rab was quantified and corresponding data were analyzed (d, e, f). The activated Rab level in untreated cells (WT) was set as 100%. Significant differences compared with NC-KD cells are shown. n = 3. * P < 0.05, ** P < 0.01

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