Fig. 1
Requirement of the uPAR84–95 sequence for SKOV-3 cell adhesion onto vitronectin. a. Viable SKOV-3 cells (2 × 105 cells/well) were seeded in triplicate in 24-well plates coated with 1% BSA (CTRL), 5 μg mL vitronectin (Vn), 5 μg mL fibronectin (Fn), 5 μg mL laminin (Lm) or 15 μg mL collagen (Coll) and allowed to adhere for 2 h. The number of adherent cells was expressed as a percentage of CTRL. Data are the means ± SD of three independent experiments with *P < 0.001. b. Cell adhesion of SKOV-3 cells in 24-well plates coated with 1% BSA (CTRL) or indicated matrix proteins in the absence (none) or presence of 2 μg/ml R3 anti-uPAR mAb, 2 μg/ml 399 anti-uPAR Ab, 2 μg/ml anti-uPAR84–95 Ab, or 2 μg/ml anti-α-tubulin Ab for 2 h. The number of adherent cells was expressed as a percentage of CTRL. Data are the means ± SD of three independent experiments with *P < 0.001. c. Adhesion of SKOV-3 cells onto Vn monitored by the xCELLigence system. Cells (1 × 104 cells/well) suspended in serum-free medium (CTRL) with or without 2 μg/ml anti-uPAR84–95 or 2 μg/ml normal rabbit serum (NRS) were seeded on Vn-coated E-plates. The impedance value of each well was automatically monitored in real-time for 2 h and expressed as a cell index value. Data represent mean ± SD from a quadruplicate experiment representative of 3 replicates