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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Targeting the Formyl Peptide Receptor type 1 to prevent the adhesion of ovarian cancer cells onto mesothelium and subsequent invasion

Fig. 2

FPR1 requirement for adhesion of SKOV-3 cells onto vitronectin. a-b. Cell adhesion of SKOV-3 cells pre-incubated with serum-free medium (none), 100 nM SRSRY (a) or 100 nM fMLF (b) for 37 min at 37 °C in humidified air with 5%CO2, onto the indicated matrix proteins. Cell adhesion was expressed as percentage of CTRL (cells adherent to plated coated with 1% BSA). Data are the means ± SD of three independent experiments with *P < 0.001. c. Adhesion onto Vn of SKOV-3 cells desensitized with diluents (none), 100 nM SRSRY, 100 nM fMLF, or 100 nM ARARY, monitored in real time for 2 h by the xCELLigence system. Data represent mean ± SD from a quadruplicate experiment representative of 3 replicates. d. Adhesion of SKOV-3 cells onto the indicated matrix proteins in the absence or presence of 10 nM RI-3. The number of adherent cells was expressed as a percentage of CTRL (cells adherent to plated coated with 1% BSA). Data are the means ± SD of three independent experiments with *P < 0.001. e. Adhesion of SKOV-3 cells onto Vn in the absence or presence of 10 nM RI-3, monitored in real time for 2 h by the xCELLigence system. Data represent mean ± SD from a quadruplicate experiment representative of 3 replicates

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