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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Targeting the Formyl Peptide Receptor type 1 to prevent the adhesion of ovarian cancer cells onto mesothelium and subsequent invasion

Fig. 5

Generation of a primary cell culture from a human high-grade serous ovarian cancer. a HGSG tissue (patient # 5) processed for IHC analysis of uPAR and FPR1 expression on paraffin sections. Nuclei were stained blue with hematoxylin. Original magnifications: × 100. b Primary EOC cells derived from the same HGSG tissue visualized by phase contrast microscopy. Original magnification: × 400. c Western blotting of whole cell lysate from EOC cells with R4 anti-uPAR mAb, anti-FPR1 Ab, and anti-GAPDH Ab. d Bar graphs showing the average quantification of the uPAR/GAPDH and FPR1/GAPDH content from 3 independent experiments. Statistical significance with *P < 0.001. e Images of human EOC cells immunostained with R4 anti-uPAR mAb or anti-FPR1 Ab and visualized by a fluorescence inverted microscope. Nuclei were stained blue with DAPI. Original magnification: 1000x. f Fluorescence associated to EOC cells pre-incubated with diluents (none), 100 nM fMLF, 100 nM SRSRY or 100 nM RI-3, for 60 min at 4 °C, and then exposed to 10 nM FITC-RI3 for additional 60 min at 4 °C. Data represent mean +/− SD from three experiments performed in triplicate with * P < 0.001. g EOC cells exposed to 10 nM FITC-fMLF or 10 nM FITC-RI-3 for 30 min at 37 °C and then visualized using a Zeiss 510 Meta LSM microscope in 2D (left) or 3D (right) projections. Original magnification: 630×

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