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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Targeting the Formyl Peptide Receptor type 1 to prevent the adhesion of ovarian cancer cells onto mesothelium and subsequent invasion

Fig. 6

RI-3 prevents EOC cell adhesion and subsequent invasion of mesothelium. a Adhesion of primary EOC cells on E-plates coated with 1% BSA (CTRL), 5 μg mL Vn, 5 μg mL Fn, 5 μg mL Lm or 15 μg mL Coll, with/without 10 nM RI-3, monitored in real time for 5 h by the xCELLigence system. Data represent mean ± SD from a quadruplicate experiment representative of 3 replicates. b-c Adhesion of EOC cells onto Vn in the absence or presence of 10 nM RI-3, monitored in real time for 3 h by the xCELLigence system. Data represent mean ± SD from a quadruplicate experiment representative of 3 replicates. Slopes represent the change rate of Cell Indexes generated in a 10–120 min time frame (c). d HPMCs were seeded in 24-well plates and allowed to attach for 20 h prior to seeding GFP-EOC cells suspended in complete medium plus diluents (none), or 10 nM RI-3 at 37 °C, 5% CO2. At the indicated times, cell associated fluorescence was assessed by a fluorescence plate reader. Data represent means ± SD of three independent experiments performed in triplicate with *P < 0.0001 vs none. e-f Mesothelium-invasion by primary EOC cells. HPMCs (5 × 103 cells/well) were seeded in E-16-well plates in growth medium and allowed to adhere for 18 h to form a confluent monolayer. Then, EOC cells (2 × 104 cells/well) were seeded in growth medium plus/minus 10 nM RI-3. Cells invasion was monitored in real-time as changes in Cell Index due to breaking of the monolayer integrity (e). Slopes represent the change rate of Cell Indexes generated in a 18–24 h time frame (f). Data represent mean ± SD from a quadruplicate experiment representative of 3 replicates

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