Fig. 2

PES1 enhances pancreatic cancer cell growth in vitro and in vivo. a-e, PANC-1 and BxPC-3 were infected with indicated constructs. After 72 h, cells were harvested for RT-qPCR analysis (a), MTS assay (b), CCK8 assay (c), BrdU assay (d) and colony formation assay (e). Data presented as Means ± SD (n = 3). *, P < 0.01; **, P < 0.01; ***, P < 0.001. f-i, pancreatic cancer cell lines (PANC-1 and BxPC-3) were transfected with indicated constructs. 72 h post-transfection, cells were injected subcutaneously into the nude mice for xenografts assay for 21 days. The image of xenografts was shown in (f), the tumor mass and volume of xenografts was determined in (g) and (h). The Ki-67 staining was shown in panel i. Data presented as Means ± SD (n = 6 for PANC-1 and n = 5 for BxPC-3). ***, P < 0.001. j-m, PANC-1 and BxPC-3 cells were transfected with indicated plasmids. After 24 h, cells were harvested for RT-qPCR analysis (j), MTS assay (k), CCK8 assay (l) and BrdU assay (m). Data was shown in the form of Means ± SD (n = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.001