Fig. 5

PES1 cooperates with BRD4 to regulate Myc expression in pancreatic cancer. a, 293 T cells were transfected with indicated plasmids. After 24 h, the whole cell lysates were harvested for Western Blotting analysis. b, the whole cell lysates of PANC-1 cells were harvested for Western Blotting analysis. c, schematic diagram depicting that a potential acetylated lysine consensus motif containing in the PES1 and the domain of BRD4. d, PANC-1 cells were transfected with indicated plasmids for 24 h. After other 24 h TSA (3 nM) treatment, cells were harvested for Western Blotting analysis. e, PANC-1 cells were transfected with indicated plasmids for 24 h. After other 24 h JQ1 (2uM) treatment, cells were harvested for Western Blotting analysis. f, PANC-1 cells were transfected with indicated plasmids for 24 h. After other 24 h JQ1 (2uM) treatment, cells were harvested for Western Blotting analysis. g, the whole cell lysates were harvested for Western Blotting analysis after transfection with indicated plasmids. h and i, PANC-1 cells were harvested for Western Blotting analysis and RT-qPCR analysis after transfection with indicated plasmids for 72 h. Data is the form of Means ± SD (n = 3). N.s., not significant; ***, P < 0.001. j and k, PANC-1 cells were transfected with empty vector (EV) or Flag-PES1 for 24 h. After other 24 JQ1 (3uM) treatment, cells were harvested for Western Blotting analysis and RT-qPCR analysis. Data is the form of Means ± SD (n = 3). N.s., not significant; **, P < 0.01; ***, P < 0.001. l and m, PANC-1 cells were infected with indicates shRNAs. 72 h post-infection, cells were harvested for Western Blotting analysis and RT-qPCR analysis. Data is the form of Means ± SD (n = 3). N.s., not significant; ***, P < 0.001. n, BRD4 ChIP-qPCR of Myc in PANC-1 cells after infected with indicated shRNA for 72 h. Data is in the form of Means ± SD (n = 3). *, P < 0.05. o, schematic diagram depicting that PES1 interacted with BRD4 to increase the expression of Myc