Fig. 1

C-2 significantly reduced the viabilities of human bladder cancer cells and induced apoptosis associated with the mitochondrial pathway. a structure of Jaspine B and C-2. b The effect of C-2 in reducing cell viabilities of bladder cancer cells (BIU87, EJ and 5637) measured by MTT assay. Cells were treated with the indicated concentrations of C-2 for 24 h and 4 μM of C-2 at indicated time points. **P < 0.02 vs. control group. c A dose-dependent induction of apoptosis by C-2 was demonstrated through flow cytometric analysis of Annexin V/PI stain assay. d Active-casepase 3, PARP and CF-PARP proteins expression were detected by western blotting at a dose- and time- dependent manner in BIU87 cells. e The mitochondria membrane potential (ΔΨ) was decreased by C-2 treatment. BIU87 cells were treated with vehicle control or varying concentrations of C-2 for 24 h. Membrane potential was measured by JC-1 dye retention using Flow Cytometry. f The protein levels of Bax, Bcl-2, XIAP and Active-caspase9 were determined by western blotting assay in BIU87 cells at indicated concentrations for 24 h