Fig. 2

C-2 induced autophagy of bladder cancer cells. a The expression levels of C-2-induced autophagy related protein Beclin-1, LC3, p62, ATG3, ATG5 and ATG7 were detected by western blotting in BIU87 and EJ cells at the indicated concentrations or treated with 4 μM of C-2 at the indicated time points. b The expression levels of C-2-induced autophagy related protein Beclin-1, p62 and LC3 were detected by western blotting in EJ cells at the indicated concentrations. c BIU87 cells were treated with 4 μM of C-2 for 12 h and 24 h. The treated and untreated samples are stained with LC3 antibody (Green) and Beclin1 antibody (Red) and DAPI (Blue) (magnification, 600X). d BIU87 cells were pretreated with LY294002 (25 μM) and Baf-A1 (0.5 μM) for 1 h and then treated with 4 μM of C-2 for an additional 24 h. Western blotting was performed to determine the effect of LY294002 and Baf-A1 on the expression of LC3-II in BIU87 cells