Fig. 3

ST6GAL1 and HOTAIR function as direct target of miR-214. a The predicted binding sites between ST6GAL1 and miR-214, and the decreased luciferease was also shown to determine the direct binding of ST6GAL1 and miR-214. b The miR-214 expression was shown in CRC tissues. c Decreased miR-214 was detected in advanced CRC stages (III + IV). d CRC patients with metastasis showed decreased miR-214. e MiR-214 level was verified in CRC cells by qRT-PCR. f The predicted binding sites between HOTAIR and miR-214 were presented, and the dual-reporter luciferase assay confirmed HOTAIR was the direct target of miR-214. g The co-location of HOTAIR and miR-214 was verified by FISH assay in CRC cells. h The co-precipitated RNA was detected by RNA immunoprecipitation assay. MiR-214 was presented as fold enrichment in Ago2 relative to IgG immunoprecipitate. i Enriched HOTAIR was shown by RIP assay. j Altered HOTAIR level was detected with the alteration of miR-214. k The ST6GAL1 mRNA and protein levels were also determined by qRT-PCR and western blot. Data were the means ± SD of triplicate determinants (*P < 0.05)