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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: The HOTAIR/miR-214/ST6GAL1 crosstalk modulates colorectal cancer procession through mediating sialylated c-Met via JAK2/STAT3 cascade

Fig. 4

HOTAIR/miR-214/ST6GAL1 axis mediates α 2, 6-sialylation of c-Met and activates JAK2/STAT3 pathway. a The ST6GAL1 level was examined by qRT-PCR in CRC cells transfected with HOTAIR or agomiR-214. b ST6GAL1 protein level was determined by western blot. c Altered FITC-SNA intensity was detected by flow cytometry of transfected cell lines. d Upregulated ST6GAL1 facilitated the SNA-binding c-Met expression of SW480 cells. e Modulation of HOTAIR and miR-214 affected SNA-binding c-Met level. f Activity of JAK2/STAT3 pathway with the treatment of c-Met antibody and SNA was measured. g The altered JAK2/STAT3 molecules with the treatment of ST6GAL1 or AMG-208. h Alteration of HOTAIR and miR-214 impacted the activity of JAK2/STAT3 signaling. Data were means ± SD of three independent assays (*P < 0.05)

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