Fig. 2

Loss of PTPRD upregulates CXCL8. a PTPRD expression in gastric cancer cell lines. b PTPRD protein and mRNA expression after lentiviral transduction to silence PTPRD. c Summary of the microarray analysis process. KEGG pathway analysis was performed on 438 differentially expressed genes (DEGs). d Loss of PTPRD induces the upregulation of CXCL8, as assessed by RT- and qRT-PCR. e CXCL8 concentration in the supernatant of cancer cells, with or without PTPRD silencing, as assessed by ELISA. f The inverse correlation between PTPRD and CXCL8 mRNA expression in TCGA data. *p < 0.05; **p < 0.01; ***p < 0.001 by unpaired Student’s t-test