Fig. 3

CXCL8 induced by the loss of PTPRD promotes angiogenesis. a-b In vitro tube formation assays were performed using MKN74 and SNU216 cells. Cancer cells were transfected with shNS or shPTPRD and then treated with control (con) siRNA or CXCL8 siRNA for 48 h. The media were then changed to supplement-free HUVEC media, cells were incubated for 24 h, and conditioned media were collected. HUVECs were incubated in conditioned media for 6–10 h and then tube formation was assessed. These data are representative of three independent experiments. *p < 0.05; ***p < 0.001 by unpaired Student’s t-test. Scale bar = 200 μm