Fig. 4

IGF2BP2 is a direct target of miR-141. a Intersections among three miRNA target prediction algorithms. b Schematic representation of IGF2BP2 3′-UTR indicating the predicted binding site of miR-141. c-e Relative expression of IGF2BP2 mRNA and protein after treatment with miR-141 mimics or inhibitors for 48 h. f and g Luciferase activity assays were performed to confirm the direct binding efficiency of miR-141 and its putative IGF2BP2 3′-UTR target. h Negative correlation between the expression levels of miR-141 and IGF2BP2 in PDAC tissues via Pearson’s correlation analysis. GAPDH served as a loading control. Data are presented as the mean ± SD of at least three independent measurements. *P < 0.05, **P < 0.01, and ***P < 0.001. P < 0.05 was considered statistically significant