Fig. 2

NFATc3 silencing upregulates DNA damage related genes. a AGS cells were infected by the indicated lentiviral vectors analyzed after infection day1 to day3. Immunoblot of the indicated antibodies were analyzed. Fold changes relative to shScr are indicated. b, c mRNA levels analyzed by qRT-PCR of NFATc3 (b) and p21 (c) in indicated AGS cells after infection day1 to day3. Statistical significance was assessed using two-tailed Student’s t-test. **p < 0.01; ***p < 0.001. d Immunoblot of DNA damage related gene set expression in that lentivirus shC3–1 or shScr infected cells. Fold changes relative to shScr are indicated. (e) γ-H2AX expression in shC3–1 and the derivative line expressing the shC3–1-resistant NFATc3 cDNA (pmax-clover-C3m). Fold changes relative to first line are indicated