Fig. 3

Arsenic sulfide suppresses NFATc3 to induce DNA damage. a Immunoblot analysis of NFATc3 and γ-H2AX expression in arsenic sulfide treated AGS, MGC803 and MKN45 cells. Fold changes relative to 0 μM are indicated. b Immunoblot analysis of NFATc3 and γ-H2AX expression in arsenic sulfide treated AGS cells. Fold changes relative to 0 h are indicated. c, d AGS cells pretreated with the pancaspase inhibitor Z-VAD-fmk (100 μM) for 1 h, then treated with arsenic sulfide for further 24 h, the viability was analyzed using flow cytometry assay (c) and Immunoblot (d). e, f Cell cycle of AGS cells infected by the indicated lentiviral vectors (e). Stacked barplot shows the fraction of cells viable in G1, S and G2/M phases of the indicated cells (f) (The percentage of cell population at G1, S, and G2/M phases are represented as mean ± S.D. of three independent experiments). g Flow cytometry analysis of apotosis in arsenic sulfide treated the indicated AGS cells. h Immunoblot analysis of NFATc3 and γ-H2AX expression in arsenic sulfide treated the indicated AGS cells. Fold changes relative to first line are indicated