Fig. 4

Arsenic sulfide increases celluar ROS and NFATc3 re-localization. a, b AGS treated with or without arsenic sulfide were stained with DCFH-DA (10 μM, 20 min, 37 °C) (a) and mean fluorescence intensity (MFI) of DCFH-DA was analyzed in each cell subset (b). Statistical significance was assessed using two-tailed Student’s t-test. **p < 0.01; ***p < 0.001. c The distribution of NFATc3 in cytoplasm and nucleus after arsenic sulfide (5 μM) treatment. Fold changes of NFATc3 protein relative to first line are indicated. d, e AGS treated with contral, arsenic sulfide (5 μM), NAC (10 mM) and arsenic sulfide plus NAC were stained with DCFH-DA (10 μM, 20 min, 37 °C) and used for ROS detection by microscopy. NAC treatment was given 1 h prior to arsenic sulfide exposure. Cells were counterstained using Hoechst (d). The distribution of NFATc3 in cytoplasm and nucleus after treated. Fold changes of NFATc3 protein relative to con are indicated (e). Fold changes of NFATc3 protein relative to first line are indicated. f, g AGS treated with or without arsenic sulfide were stained with DCFH-DA (10 μM, 20 min, 37 °C) (f) and MFI of DCFH-DA was analyzed in each cell subset (g). Statistical significance was assessed using two-tailed Student’s t-test. *p < 0.05; **p < 0.01