Fig. 5

NFATc3 silencing and arsenic sulfide treatment upregulate RAG1. a The Venn diagram displays overlaps among LogFC ≥2 genes in response to shC3 treatment in the AGS-shC3 day2 (blue), AGS-shC3 day3 (orange) and MKN45-shC3 day2 (green). b Heatmap of 22 genes significantly modulated in indicated cell lines. c qRT-PCR analysis of RAG1 expression in lentivirus shC3–1 or shScr infected AGS cells for the indicated time points. Statistical significance was assessed using two-tailed Student’s t-test. ***P < 0.001. d Immunoblot analysis of RAG1 expression in lentivirus shC3–1 or shScr infected AGS cells for the indicated time points. Fold changes relative to shScr are indicated. e Immunoblot analysis of RAG1 and γ-H2AX expression in RAG1-overexpressed 293 T cells. Fold changes of γ-H2AX protein relative to con are indicated. f Immunoblot analysis of RAG1 expression in arsenic sulfide treated AGS cells. Fold changes relative to first line are indicated. g qRT-PCR analysis of RAG1 expression in arsenic sulfide treated AGS cells. Statistical significance was assessed using two-tailed Student’s t-test. ***p < 0.001. h Immunoblot analysis of γ-H2AX expression in AGS cells which RAG1 and shC3–1 both knockdown. Fold changes relative to first line are indicated