Fig. 2

PI3K-C2β downregulation delays cell division. a PC3 cells were transfected with the indicated siRNAs. After 48 h, cells were monitored by time-lapse microscopy for 19 h. Representative images of si control-transfected PC3 cells acquired at the indicated minutes are shown. Arrows indicate the intercellular bridges. Graphs indicate the time required by each cell to progress from cell rounding to complete separation of daughter cells (from i to viii), from cell rounding to splitting into two cells (from i to iii) and from the appearance of the two daughter cells to their complete separation (from iii to viii). Data are from n = 3 [si control, si PI3K-C2β (2)], n = 2 [si PI3K-C2β (1)] and n = 2 [si PI3K-C2β (3)] independent experiments performed in duplicate. The total numbers of cells analysed were as follows: 598 (si control), 29 [si PI3K-C2β (1], 447 [si PI3K-C2β (2)] and 161 [si PI3K-C2β (3)]. **p < 0.01, ***p < 0.001, ****p < 0.0001 vs si control (two tailed, unpaired t-Test with Welch’s correction). b HeLa cells overexpressing RFP-α-tubulin were transfected with the indicated siRNAs. Efficient downregulation of PI3K-C2β was confirmed by Western blotting. GAPDH was used as loading control. After 24 h, cells were monitored by time-lapse microscopy for further 20 h. Graphs indicate the time required by each cell to progress from prophase to abscission. ***p < 0.001