Fig. 4

Combination of PI3K-C2β downregulation and docetaxel treatment strongly inhibits 2D colonies of PC3 cells in clonogenic assays. PC3 cells and stable cell lines (a, b) were plated as single cells in 6 well plates (200 cells/well). Alternatively, PC3 were transfected with the indicated siRNAs or treated with transfection reagent alone (oligo). Non-transfected (NT) cells were also used as additional control cells. After 48 h, cells were detached and plated as single cells (c). Cells were incubated in complete media for 10 days in the presence of the indicated concentrations of docetaxel (or vehicle, DMSO) before being fixed and stained with crystal violet. Representative images of 2D colonies from PC3 and stable cell lines at the end of the experiment are shown in (a). Data in (b) and (c) indicate the number of colonies/well (>65 cells) and are means ± s.e.m. of n = 3 independent experiments performed in duplicate. In (b): *p < 0.05, **p < 0.01 vs corresponding PC3; ^#p < 0.05 vs corresponding sh scrambled (3); ^$p < 0.05 vs corresponding sh scrambled (4) (two tailed, unpaired t-Test with Welch’s correction). In (c): **p < 0.01, ***p < 0.001 vs corresponding NT; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs corresponding oligo; ^$p < 0.05, ^$$p < 0.01, ^$$$p < 0.001 vs correspondent si control (two tailed, unpaired t-Test with Welch’s correction)