Fig. 5

Combination of PI3K-C2β downregulation and docetaxel treatment strongly inhibits 2D colonies of HeLa cells in clonogenic assays. HeLa cells were transfected with siRNAs targeting PI3K-C2β, a control siRNA (si control) or treated with transfection reagent alone (oligo). Additional control cells were not transfected (NT). Cells were detached 24 h post transfection and plated as single cells (100 or 200 or 400 cells/well) in 6 well plates. Cells were incubated in complete media for 7 days in the presence of the indicated concentrations of docetaxel (or vehicle, DMSO) before being fixed and stained with crystal violet. Representative images of 2D colonies at the end of the experiment are shown in (a). Data in (b) indicate the number of colonies/well expressed as percentage of colonies from NT cells treated with DMSO and are means ± s.e.m. of n = 5 independent experiments performed in duplicate. **p < 0.01, ***p < 0.001 vs corresponding NT; ^##p < 0.01, ^###p < 0.001 vs corresponding oligo; ^$p < 0.05, ^$$p < 0.01, ^$$$p < 0.001 vs corresponding si control