Fig. 3

ESCC cells deliver ZFAS1 to surrounding cancer cells by exosomes. a: The morphological characteristics of exosomes which was round or oval membranous vesicle were observed by a TEM. b: Exosomes surface marker protein CD63, CD9 and CD81 detected by western blot analysis. c: Laser confocal observed fluorescence labeled exosomes and exosome uptake (× 400), the expression of ZFAS1 in receptor cells detected by RT-qPCR. d: The expression of ZFAS1 in donor Eca109 cells, exosomes and recipient Eca109 cells determined by RT-qPCR. Measurement data were depicted as mean ± standard deviation, and comparisons among multiple groups were assessed by one-way analysis of variance followed by Tukey’s post hoc test; Repetitions = 3 in cellular experiment