Fig. 4

LncRNA ZFAS1 acted as a sponge to regulate miR-124. a: FISH assay was performed to verify the ZFAS1 subcellular localization. b: Prediction of binding site between ZFAS1 and miR-124 in RNA22 website. c: Dual luciferase reporter gene assay was conducted to detect the combination of ZFAS1 and miR-124. d: The combination of ZFAS1 and Ago2 detected by RIP assay. e: Enrichment of ZFAS1 by miR-124 detected by RNA pull-down assay. f: Expression of miR-124 in each group detected by RT-qPCR. g: Prediction of binding site between miR-124 and STAT3 in RNA22 website. h: The combination of miR-124 and STAT3 detected by dual luciferase reporter gene assay. i: STAT3 expression in each group detected by RT-qPCR. * p < 0.05. Measurement data were depicted as mean ± standard deviation, comparisons between two groups were conducted by independent sample t-test, and comparisons among multiple groups were assessed by one-way analysis of variance followed by Tukey’s post hoc test; Repetitions = 3 in cellular experiment