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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Pre-metastatic niche triggers SDF-1/CXCR4 axis and promotes organ colonisation by hepatocellular circulating tumour cells via downregulation of Prrx1

Fig. 2

Prrx1, as a downstream factor of SDF-1, contributes to lung colonisation of HCC cells. a Western blotting of Prrx1 expression in SDF-1-treated cultured cells and control cells. b Protein and mRNA expression of Prrx1 in 5 HCC cell lines detected by western blot and RT-qPCR, respectively. c Bel-7404 cells were transduced with Prrx1 shRNAs. Western blot analysis of PRRX1 in two groups is shown. The signal was quantified using densitometric scanning software, and the protein abundance was normalised to β-actin. d Nude mice xenografted with indicated cells. Kaplan-Meier survival curves and univariate analyses (log-rank) for the xenografted mouse model are shown. e Representative photographs of haematoxylin and eosin-stained and PRRX1-stained colonisation nodules of the lung are shown. f IHC of PRRX1 in primary foci and lung metastatic sites of HCC patients.

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