Fig. 2

NDRG1 could increase p21 expression in colorectal cancer. a and (b) Changes in p21 expression after NDRG1 overexpression and knockdown in HCT116 and SW1116 cell lines. GAPDH was used as loading control for whole cell lysates. Densitometry represents the expression of the proteins relative to GAPDH. c Representative images of p21 expression by immunofluorescence staining (red: p21; blue: nuclear (DAPI), scale bar: 50 μm and 20 μm for SW1116 and HCT116, respectively). Error bar represents the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001