Fig. 3From: N-myc downstream-regulated gene 1 inhibits the proliferation of colorectal cancer through emulative antagonizing NEDD4-mediated ubiquitylation of p21NDRG1 inhibited cell proliferation through increasing p21 expression in colorectal cancer. a and (b) Endogenous p21 was knocked down after transfection with si-RNA-p21. Colony formation assay (c), CCK-8 assay (d), and cell cycle analysis (e) were performed to demonstrate that p21 knockdown could rescue the inhibitory effect on CRC cell proliferation induced by NDRG1 overexpression. Error bar represents the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001Back to article page