Fig. 4

MFAP5 facilitated ICC cell cycle transition. a, c Cell cycle flow cytometry analysis showed the different G0/G1 percentage after co-cultured with recMFAP5 and after transfected sh-MFAP5. b, d Three replicate experiments on cell cycle analysis. e Western blot assay showed the expression level of CCND1, CDK4, CDK6 CDC25A, P21 in ICC cells line after co-cultured with recMFAP5 and after transfected sh-MFAP5. g, h RT-PCR results showed the relative expression level of cell cycle genes in sh-MFAP5 ICC cell lines. *P < 0.05,**P < 0.01,***P<0.001