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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: MFAP5 facilitates the aggressiveness of intrahepatic Cholangiocarcinoma by activating the Notch1 signaling pathway

Fig. 5

MFAP5 positively regulated Notch1 pathway transcription. a Heat map showed results of RNA-seq of SSP-25 and RBE transformed sh-control, sh-MFAP5–1# and sh-MFAP5–2#. b, c GSEA results showed down-regulation of Notch pathway in cells transfected sh-MFAP5 compared with sh-Control. d, e RT-PCR results showed the mRNA level of genes in NOTCH1 pathway after transfected sh-MFAP5 in ICC cell lines. f, g Western blot analysis of Notch1 pathway target genes in sh-MFAP5 and sh-Control cells (f) and co-culture with recMFAP5 and control cells (G, each “+” represented 50μg/ml recMFAP5). h, i Co-IP results showed that MFAP5 could bind directly with NOTCH1. *P < 0.05,**P < 0.01,***P<0.001

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