Fig. 6

Chromatin Accessibility by ATAC-seq Analysis Reveals a Role of MFAP5 for NOTCH1 Activation. a, b CCK-8 assay showed the proliferation differences of ICC cells co-cultured with recMFAP5 and combination (recMFAP5 + FLI-06). c Western blot results of cell cycle genes in ICC cell lines after co-cultured with recMFAP5 and FLI-06. d ATAC-seq heatmap results showed the appearing peaks status in different groups. e GSEA analysis revealed genes in the G1/S phase following recMFAP5 co-culture. f The gene peaks results revealed changes in different groups in RBE cell line. g Proposed schematic models illustrated MFAP5 facilited the aggressiveness of ICC via modulating Notch pathway/G1S signaling axis