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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: RNA-binding protein KHSRP promotes tumor growth and metastasis in non-small cell lung cancer

Fig. 4

KHSRP physically interacted with HNRNPC in NSCLC cells. a A diagram showing tandem affinity purification (TAP) and mass spectrometry analyses of KHSRP-associated proteins. b Silver staining was used to detect immunoprecipitated Flag-HA-KHSRP-binding proteins. c A list of representative proteins that interact with the KHSRP protein is shown. d The interaction between KHSRP and HNRNPC detected by mass spectrometry analysis was verified by immunoprecipitation in HEK293T cells transfected with plasmids encoding KHSRP or HNRNPC. e The interaction between endogenous KHSRP and HNRNPC was detected by immunoprecipitation in NCI-H1299 and A549 cells. f Immunofluorescence staining was used to detect the colocalization and coexpression of the KHSRP and HNRNPC proteins in the nucleus. g The regulatory relationship between KHSRP and HNRNPC was verified by Western blotting. h A549 cells with KHSRP knockdown and NCI-H292 cells overexpressing KHSRP were treated with CHX (100 μg/ml) for the indicated time points. The cell lysates were examined by Western blot

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