Fig. 5
From: RNA-binding protein KHSRP promotes tumor growth and metastasis in non-small cell lung cancer
HNRNPC promoted NSCLC cell proliferation, migration and invasion. a The levels of HNRNPC expression in 4 human NSCLC cell lines were measured by real-time PCR and Western blot. b The mRNA and protein levels of HNRNPC in HNRNPC knockdown and negative control A549 cells and in HNRNPC overexpression and negative control NCI-H1299 cells were determined by real-time PCR and Western blotting analyses, respectively. c The effect of KHSRP on cell proliferation was evaluated by a CCK-8 assay in A549, NCI-H1299 and NCI-H292 cells. d The role of KHSRP in cell proliferation was evaluated by clone formation assays in A549, NCI-H1299 and NCI-H292 cells. e The effect of HNRNPC on cell migration was assessed by Transwell migration assays in A549, NCI-H1299 and NCI-H292 cells. f The effect of HNRNPC on cell invasion was assessed by Matrigel invasion assays in A549, NCI-H1299 and NCI-H292 cells. g Wound-healing assays were performed in A549, NCI-H1299 and NCI-H292 cells