Fig. 1

IRI induced more severe HCC recurrence in fatty liver. a, b Representative images and hepatic replacement area (HRA) of HCC recurrence in CD and HFD mice inoculated with Hepa1–6 cells with or without IRI. Scale bars, 1 cm. (n = 3–4 per group) c Representative H&E staining of liver sections in CD and HFD mice inoculated with Hepa1–6 cells with or without IRI. Scale bars, 200 μm. d qPCR analysis of Bv8, S100a8, S100a9 and VEGF in CD and HFD feeding mice after IRI. (n = 4–5 per group) e qPCR analysis of Bv8, S100a8, S100a9 and VEGF in AML12 cells stimulated with PA (50 μM) followed by HR. (n = 4–5 per group) Data are mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired Student’s t- test