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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Ischemia reperfusion injury promotes recurrence of hepatocellular carcinoma in fatty liver via ALOX12-12HETE-GPR31 signaling axis

Fig. 4

12-HETE enhanced migration of HCC cells via activation of EMT. a, c Immunoblot analysis of E-cadherin, N-cadherin, Vimentin, Snail and Slug in Hepa1–6 cells stimulated with 12-HETE. Protein levels were normalized to GAPDH and analyzed. (n = 3 per group) b, d. Immunoblot analysis of E-cadherin, N-cadherin, Vimentin, Snail and Slug in Hepa1–6 cells stimulated with supernatant of AML12 cell. Protein levels were normalized to GAPDH and analyzed. (n = 3 per group) e-h. Immunoblot analysis of E-cadherin, N-cadherin, Vimentin, Snail and Slug in bel-7402 and Huh7 cells stimulated with 12-HETE. Protein levels were normalized to GAPDH and analyzed. (n = 3 per group) i, j. qPCR analysis of E-cadherin, N-cadherin, Vimentin, Snail and Slug in bel-7402 and Huh7 cells stimulated with 12-HETE. (n = 4–5 per group) k-m. Representative immunofluorescence staining of Vimentin in bel-7402, Hepa1–6 and Huh7 cells. Scale bars, 100 μm. Data are mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired Student’s t- test

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