Fig. 5

12-HETE enhanced migration of HCC cells via activation of MMPs. a, c Immunoblot analysis of MMP2, MMP7, MMP9 and MMP13 in Hepa1–6 cells stimulated with 12-HETE. Protein levels were normalized to GAPDH and analyzed. (n = 3 per group) b, d Immunoblot analysis of MMP2, MMP7, MMP9 and MMP13 in Hepa1–6 cells stimulated with supernatant of AML12 cell. Protein levels were normalized to GAPDH and analyzed. (n = 3 per group) e-h Immunoblot analysis of MMP2, MMP7, MMP9 and MMP13 in bel-7402 and Huh7 cells stimulated with 12-HETE. Protein levels were normalized to GAPDH and analyzed. (n = 3 per group) i, j. qPCR analysis of MMP2, MMP7, MMP9 and MMP13 in bel-7402 and Huh7 cells stimulated with 12-HETE. (n = 4–5 per group) k-m Representative immunofluorescence staining of MMP9 in bel-7402, Hepa1–6 and Huh7 cells. Scale bars, 100 μm. Data are mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired Student’s t- test