Fig. 6

12-HETE induced EMT and MMPs through activation of PI3K/AKT/NF-κB pathway. a-d Immunoblot analysis of PI3K, AKT and NFκB in bel-7402 and Huh7 cells stimulated with 12-HETE. Protein levels were normalized to GAPDH and analyzed. (n = 3 per group) e, f Immunoblot analysis of PI3K, AKT, NFκB, E-cadherin, N-cadherin, Vimentin, Snail, Slug, MMP2, MMP7, MMP9 and MMP13 in bel-7402 and Huh7 cells stimulated with LY294002 and 12-HETE. g, h Representative immunofluorescence staining of Vimentin and MMP9 in bel-7402 cells. Scale bars, 100 μm. Data are mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired Student’s t- test