Fig. 1

Neuroblastomas express NF-YAx. a Illustration of NF-YA glutamine-rich transactivation, serine threonine-rich (S/T), interaction (ID) and DNA binding (DBD) domains with relative positions and amino acid sequences of exons B and D, in NF-YAl and NF-YAx isoforms. Southern blots showing NF-YA isoform and GAP RT-PCR levels in: b primary stage 1–4 human NBs; c NB cell lines and human embryonic neural SC (left panel) and d HEK-293 and ST14A cells (right panel), hybridized with NF-YA exon C (upper panels) and GAP (lower panels) probes (* indicates NF-YAx). e Western blot showing NF-YAl and NF-YAs protein levels in SH-SY5Y and SHEP extracts (30 μg). f Autoradiographs demonstrating in vitro translated NF-YAl, NF-YAs, NF-YAx (left panel), NF-YB and NF-YC proteins (right panel)