Fig. 4

NF-YAx is cytotoxic. a Sterile purified pcDNA, NF-YAl, NF-YAs, NF-YAx and NF-YAdn plasmids used in transfections plus RT-PCR plus Western blots demonstrating NF-YAl, NF-YAs, NF-YAx and GAP mRNA and protein levels in HEK-293 cells, following 48 h transfection with pcDNA3.1 NF-YAl, NF-YAs, NF-YAx and empty pcDNA mammalian expression vectors. b Representative Micrographs illustrating ST14A (upper 6 panels), SH-SY5Y (middle 6 panels) and HEK-293 (lower 6 panels) cell-death, following 48 h transfection with pcDNA, NF-YAl, NF-YAs, NF-YAx and NF-YAdn expression vectors (bar = 100 μm) plus histograms demonstrating the percentage change in adherent and suspension (dead/dying) ST14A (upper), SH-SY5Y (middle) and HEK-293 (lower) cells, following 48 h transfection with pcDNA, NF-YAl, NF-YAs, NF-YAx and NF-YAdn expression vectors, results are displayed as mean (±SD) percent in three independent assays performed in duplicate (* = significant). c Representative AO/EtBr assays (bar = 100 μm) demonstrating HEK-293, SH-SY5Y and ST14A suspension cell death (orange/red), 48 h following transfection with NF-YAx and NF-YAdn expression vectors plus a histogram of the percentage of suspension cell-death, displayed as mean (± SD) in three independent AO/EtBr assays performed in duplicate. d Micrographs demonstrating lack of stable TrkAIII transfected SH-SY5Y cell-death, following 48 h transfection with either NF-YAl, NF-YAs or NF-YAx expression vectors (bar = 100 μm)