Fig. 9

PTC-209 abrogates SH-SY5Y proliferation. a Phase contrast micrographs (bar = 100 μm) and histograms demonstrating abrogation of parental SH-SY5Y (NT- SH-SY5Y), stable NF-YAl, NF-YAs, NF-YAx1 and NF-YAx2 SH-SY5Y-transfectant proliferation, following 24 h treatment with 10 μM PTC-209. Line graph data are displayed as mean (±S.D.) fold change in cell number compared to 0-h controls, in three experiments performed in duplicate (* = significant). b RT-PCRs demonstrating no induction of KIF1Bβ and no reduction in Bmi-1 expression in stable pcDNA, NF-YAl, NF-YAs, NF-YAx1 and NF-YAx2 SH-SY5Y-transfectants, following 24 h treatment with 10 μM PTC-209