Fig. 2
From: HOXC-AS1-MYC regulatory loop contributes to the growth and metastasis in gastric cancer
Knockdown of HOXC-AS1 inhibited cell proliferation, migration, invasion and EMT in GC. (a) qRT-PCR result of HOXC-AS1 in BGC-823 and AGS cells transfected with shCtrl or two shRNAs aiming at HOXC-AS1. (b) The viability of BGC-823 and AGS cells under above transfections was assessed by CCK-8 assays. (c, d) EdU and TUNEL assays were respectively performed to evaluate cell proliferation and apoptosis in BGC-823 and AGS cells with HOXC-AS1 inhibition or not. (e, f) Transwell assays were implemented to estimate the effect of HOXC-AS1 on GC cell migration and invasion. Western blot analysis of E-cadherin, N-cadherin and Vimentin in BGC-823 and AGS under HOXC-AS1 knockdown versus control. ** P < 0.01