Fig. 4

HOXC-AS1 triggered MYC expression in GC by absorbing miR-590-3p in cytoplasm. (a) qRT-PCR result of MYC level in GC cells with or without HOXC-AS1 silence. (b) DIANA predicted that there was only two miRNA that interacted with both HOXC-AS1 and MYC. (c) The expression levels of above two miRNAs in GC cell lines were determined by qRT-PCR. (d) qRT-PCR result of the two miRNAs in BGC-823 and AGS cells with HOXC-AS1 inhibition or not. (e) RIP assay proved the co-existence of HOXC-AS1, miR-590-3p and MYC mRNA in RISC. (f, g) Luciferase reporter assay showed the competition binding of HOXC-AS1 and MYC mRNA to miR-590-3p. (H) Relative expression of MYC in indicated BGC-823 and AGS cells was analyzed via qRT-PCR. * P < 0.05, ** P < 0.01