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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: CircNFIX promotes progression of glioma through regulating miR-378e/RPN2 axis

Fig. 3

circNFIX is a sponge of miR-378e. (a) StarBase database predicted the binding sites of circNFIX and miR-378e. (b-e) Luciferase reporter assay and RIP assay were performed in T98 and U251 cells to confirm the association between circNFIX and miR-378e. (f and g) RNA pull-down assay was performed in T98 and U251 cells to confirm the association between circNFIX and miR-378e. (h) The expression level of miR-378e was measured in glioma cells and normal astrocyte cell line. (i and j) The abundances of miR-378e in T98 and U251 cells were detected after transfection of pcDNA, circNFIX, si-NC or si-circNFIX. *P < 0.05, ***P < 0.001 compared with indicated control group

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