Fig. 5
ATRA effects on the levels of cardiolipins. a Biological triplicates of the indicated breast cancer cells were treated with vehicle (DMSO) or ATRA (10- 6 M) for 48 h. Left: The box plots show the median ± SD levels of cardiolipins (CLs). The number of different CL molecules identified by mass-spectrometry is indicated in parenthesis. Luminal cell-lines are marked in red and basal cell-lines are marked in blue. The luminal and basal cell-lines are ordered according to decreasing sensitivity to the anti-proliferative effect of ATRA from left to right, as indicated (decreasing ATRA-score). Right: The diagram indicates the correlations between the ATRA/DMSO ratio of the mean values calculated for CLs in each cell-line and the corresponding ATRA-score. b Biological triplicates of SK-BR-3 cells were treated with vehicle (DMSO) or ATRA (10- 6 M) for the indicated amounts of time. The box plot shows the median ± SD levels of cardiolipins (CLs). c Biological triplicates of SK-BR-3 cells were treated with vehicle (DMSO) or the indicated concentrations of ATRA for 48 h. The box plot shows the median ± SD levels of cardiolipins (CLs). *Significantly different (p < 0.05) from the corresponding vehicle treated control using the Student’s t-test. **Significantly different (p < 0.01) from the corresponding vehicle treated control using the Student’s t-test