Fig. 3

miR-205 targets and negatively regulates RHPN2. a predicted binding sites of miR-205 in the 3’UTR of RHPN2; b binding of miR-205 to RHPN2 confirmed by dual-luciferase reporter gene assay; c RHPN2 mRNA expression in cells after overexpression or inhibition of miR-205 treatment detected by RT-qPCR; d RHPN2 protein expression in cells after overexpression or inhibition of miR-205 treatment detected by using western blot analysis; *, p < 0.05. Measurement data were expressed as mean ± standard deviation; comparisons between two groups were analyzed using unpaired t-test; the experiment was repeated three times independently